| BD UltraPool™ HLM 150
enables consistency in experimental results in multi-site and long-term programs by delivering reproducible data across multiple CYP and UGT enzymes as compared to smaller pools of donors.
The new BD UltraPool HLM 150 is the first
commercially available large donor pool of
150 donors statistically modeled to provide
researchers with a high degree of lot-to-lot
consistency for CYP and UGT enzyme activity,
and to naturally represent the “average patient”
and known CYP polymorphisms.
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High degree of lot-to-lot consistency for CYP and UGT activity
BD UltraPool HLM 150 pool is statistically modeled so that lot-to-lot variability represented
as coefficient of variation (CV) is < 10% (mean) for the more variable CYP enzyme
(CYP2C19), and < 5% (mean) for the key drug metabolizing CYPs (CYP1A2, CYP2C9,
CYP2D6 and CYP3A4). This lot-to-lot consistency is critical for researchers to achieve data
consistency for metabolic stability, CYP inhibition and reaction phenotyping assays year
after year with multiple HLM lots.
Natural representation of the “average patient” and known CYP polymorphisms
The large donor pool (150 donors, 300 alleles for diploid genes), consisting of an equal
donor mix on a per milligram microsomal protein basis provides the best representation of
an “average patient” drug metabolism activity. BD UltraPool HLM 150 provides consistency
and confidence in the assay data across a wide selection of discovery screening and
development assays.
Large lots available for multi-year drug discovery programs
The BD UltraPool HLM 150 donor pool size is prepared from donors where considerable
tissue is available, and is manufactured in large lot sizes. This supports multi-year and
multi-site programs by providing data consistency between ADME discovery screening
and development groups. This donor pool size is specifically designed to serve the entire
research organization - from drug discovery to drug development.
Illustration of the actual enzyme activity level for CYP1A2 in the BD UltraPool HLM 150
first lot and the calculated mean, upper and lower limits for a 150 donor pool based on
Monte Carlo simulations. The bars are (from left to right): the overall mean, the 95% lower
confidence limit, and the 95% upper confidence limit calculated based on the CV from
Monte Carlo simulations for a 150 donor pool, and the measured CYP1A2 activity in the
first lot of BD UltraPool HLM 150.
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Send us your questions about the new
BD UltraPool HLM 150 large donor pool —we're ready to answer.
Learn about optimizing donor numbers for consistent pooled human liver microsomes.
Visit our online catalog to learn more about the BD UltraPool HLM 150. For additional product information please download the BD UltraPool HLM 150 product information sheet [PDF].
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