Bioimaging Applications for Cell Biology
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The power and flexibility of the BD Pathway™ system enables researchers to develop and perform a wide array of high-content biological applications.
Our applications take advantage of our live-cell kinetic and confocal capabilities, and can be run in multi-plate high-throughput and/or low-throughput modes, depending on the need.
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Imaging applications can be divided into the following four assay modes:
- Measurement of fluorescence intensity
- Fluorescence distribution
- Morphological changes in fluorescently labeled structures
- Migration of fluorescently labeled cells
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Assay Modes
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Fluorescence intensity |
Fluorescence distribution and colocalization |
Morphology |
Cell Migration |
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When to use
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- Biological response appears as a change in fluorescence intensity but other fluorescence detection methods are ineffective
- Few cells are available (e.g. primary cells, stem cells)
- Cell population is heterogeneous
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- Total fluorescence does not change within a cell following activation
- Identify where within a cell an event occurs
- Colocalize cellular events
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- Biological response measured by changes in cell size or shape
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- Biological response is measured as cellular movement
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Example of BD Pathway application
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- Calcium kinetics
- Mitotic index
- Live/dead
- Cell viability
- Cytotoxicity
- Cell cycle
- Apoptosis
- Mitochondrial health (JC-1,TMRE, MitoTracker)
- Steatosis
- IkB degradation
- Reactive oxygen species
- Whole organism imaging (c. elegans, arabidopsis, zebrafish)
- Tissue arrays
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- Cytoplasmic/ nucleus translocation assays
- DNA damage (and repair)
- Mitotic defects (monopolar spindles)
- Apoptosis
- Synaptic junction localization
- FISH
- 3-D cell imaging (stem cells, pancreatic islets)
- Transfluor® GPCR (receptor internalization)
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- Tube formation (angiogenesis)
- Neurite outgrowth
- Micronucleus assay
- Morphometric analysis (tubulin)
- Apoptosis (nuclear size, granularity)
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