BD ACTOne Technology
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A cAMP biosensor for Gs and Gi GPCR high-throughput drug screening and profiling, BD ActOne™ technology is easy to scale.
The BD ACTOne™ platform is a highly-sensitive and cost-effective tool for screening G-protein coupled receptors (GPCRs) that signal through Gs or Gi G-proteins. Using a natural biosensor approach, this technology has many advantages over biochemical or other live cell technologies.
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GPCR Pathway
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BD ActOnetechnology offers:
- Measurement of physiological levels of cAMP in living cells approximate to plasma membrane
- Excellent signal to noise ratio and reproducibility for better data confidence
- Stable signal allowing kinetic or endpoint assay formats
- Screening of agonists and antagonists (or allosteric modulators), saving time and reagents
- Experimentation with endogenous receptors
- Familiar protocols and instrumentation to simplify validation and shorten integration time
- A powerful deorphanizing tool to help study novel receptors
- Compatibility with single-cell imaging platforms for high-content analysis and multiplexing
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