| Suitable for short- and long-term growth and differentiation of a variety of cell types, including epithelial cells (e.g. hepatocytes), neurons, endothelial cells, osteoblasts, chondrocytes, fibroblasts, and smooth muscle cells. |
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The BD™ 3D Collagen Composite Scaffold is a natural scaffold manufactured from a proprietary mixture of collagens that are derived from bovine hide. Overall, this material exhibits collagen fibrillar architecture, which is representative of the structure of collagen within the interstitial matrix. |
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The BD™ 3D OPLA® (Open-Cell Polylactic Acid) Scaffold is a synthetic polymer scaffold that is synthesized from D,D-L,L polylactic acid. This material has a facetted architecture, which is effective for culturing high density cell suspensions. |
Current research in the field of tissue engineering is focused on the development of appropriate tools and methods for promoting the repair or regeneration of biological tissues.[ 8-17 ] This work involves both in vitro and in vivo analyses of living cells with or without acellular three-dimensional (3D) biomaterials. A central objective of this research is to identify cellular environments that support a desired cellular behavior such as proliferation or differentiation.
The BD™ 3D Collagen and OPLA® scaffolds are suitable for short- and long-term growth and differentiation of a variety of cell types, including epithelial cells (e.g., hepatocytes { 1 }), neurons, endothelial cells [ 7 ], osteoblasts [ 4 ], chondrocytes [ 3,5,6 ], fibroblasts, and smooth muscle cells [ 2 ]. The 3D structure mimics the in vivo environment to induce tissue formation or promote tissue repair. An optimized assay and protocol will enable you to perform your experiments with complete confidence.
For more information please download the brochure, under Technical Documents section below.
The BD™ 3D Collagen Composite and OPLA® Scaffolds were used to examine the in vitro growth and differentiation of MC3T3-E1 osteoblasts. These cells have been used to evaluate stage specific growth and differentiation of osteoblasts in 2D and 3D cultures [10,11]. When MC3T3-E1 cells were cultured on the BD 3D Collagen Composite and OPLA Scaffolds, cell growth increased dramatically within 1-2 days and was maintained for up to 10 days in culture (Figure 1). The increase in cell growth was found to correlate with an increase in cellular DNA content (Figure 2). In addition, increased cell growth correlated with elevated levels of alkaline phosphatase activity (Figure 3), which is a marker for osteoblast differentiation. Taken together, these findings indicate that the BD 3D Collagen Composite and OPLA Scaffolds provide a suitable environment for in vitro analyses of osteoblast growth and differentiation.
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| Figure 1: The BD™ Oxygen Biosensor System was used to analyze cellular growth. The consumption of oxygen by proliferating cells was measured by fluorescence detection, which is expressed in normalized relative fluorescence units (NRFU). |
Figure 2: Cellular DNA content was measured using the Picogreen Assay Kit (Molecular Probes). |
Figure 3: To assess osteoblast differentiation, alkaline phosphatase activity was measured using the AP Assay Kit (Sigma Diagnostics). |
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Data was provided by Dr. Mohammed Heidaran, BD Technologies.
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<<Click on image to enlarge>> |
| 3D Structure |
Mimics the in vivo environment to induce tissue formation or promote tissue repair |
Proprietary Collagen Composite
and OPLA® Substrate |
Supports short- and long-term growth and differentiation of a variety of cell types, including epithelial cells (e.g., hepatocytes), neurons, endothelial cells, osteoblasts, chondrocytes, fibroblasts and smooth muscle cells |
| Optimized Assay and Protocol |
Perform your experiments with complete confidence |
- BD™ 3D Collagen Composite and OPLA® Scaffolds - Brochure [ PDF ]
- BD™ 3D Collagen Composite Scaffold - Guidelines for Use[ PDF ]
- BD™ 3D OPLA® Scaffolds - Guidelines for Use[ PDF ]
- BD™ 3D Biodegradable Scaffolds - Frequently Asked Questions [ PDF ]
- Non-Invasive Monitoring of Oxygen Consumbion by 3-Dimensional Tissue Constructs[ PDF ]
- Oxygen Consumption of 3D Cell Constructs as a Nondestructive Predictor of Growth and Differentiation - Poster [ PDF ]
References
- Takezawa, T. et al. Tissue Engineering, 6:641-650 (2000)
- Kim, B-S. et al, Experimental Cell Research. 251: 318-329 (1999)
- Chu, C.R. et al., Biomaterials. 16:1381-1384. (1995)
- Malekzadeh, R. J. Periodental. 1256-1262 (1998)
- Chu, C.R. et al., J. Biomedical Materials Research. 29:1147-1154 (1995)
- Chu, C.R. et al. Clinical Orthopaedics and Related Research. 340: 220-229 (1997)
- Sheridan, M.H. et al., Journal of Controlled Release. 64: 91-102 (2000)
- Bottaro, D.P. and Heidaran, M.A., Engineered Extracellular Matrices; Biological Solution for Tissue Repair, Regeneration and Disease Modification, e-biomed: the journal of regenerative medicine, 2, http://www.liebertpub.com/EBI/default1.asp (2001).
- DeWitt, N.(ed.), Nature, 414: 87-131 (2001).
- Freed, L.E., et al., Bio/Technology, 12: 689-693 (1994).
- Hoffman, R.M., Stem Cells, 11: 105-111 (1993).
- Lagasse, E., et al., Immunity 14: 425-436 (2001).
- Langer, R. 1994 Whitaker Lecture: Polymers for Drug Delivery and Tissue Engineering. Annals of Biomedical Engineering, 23: 101-111 (1995).
- Lanza, R.P., et al., Principles of Tissue Engineering, R.G. Landes Company: Austin, TX (1997).
- Liebmann-Vinson, A., et al., Bioactive Extracellular Matrices: Biological and Biochemical Evaluation, in Tissue Engineering and Biodegradable Equivalents. Lewandrowski et al. (eds). Marcel Dekker, Inc.: New York (2002).
- Mooney, D.J. and Mikos, A.G., Scientific American, 280: 60-65 (1999).
- Stock, U.A. and Vacanti, J.P., Annu. Rev. Med. 52: 443-451 (2001).
OPLA is a registered trademark of Kensey Nash Corporation.
The OPLA material is manufactured under one or more of the following U.S. Patents: 4,186,448; 5,736,160; 5,855,608; 5,935,594; 5,981,825; 6,005,161; and 6,264,701.
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