BD APC-H7 Photostable Tandem Conjugates
BD™ APC-H7 from BD Biosciences offers a new level of stability, making it easier to achieve reliable results across a broad range of multicolor flow cytometry applications.
Figure 1. BD APC-H7 demonstrated stability and reduced spillover.
The experiments shown here compare the stability, as measured by % spillover, of whole blood stained with BD APC-H7 and APC-Alexa Fluor® 750 conjugated antibodies. Stained samples were stored in 1% PFA at room temperature under ambient light.
More reliable and reproducible
New BD APC-H7 is more stable and less affected by light, temperature, and formaldehyde-based fixatives than other APC-cyanine tandem dyes such as APC-Cy™7 and APC-Alexa Fluor® 750. BD APC-H7 also is engineered to minimize spill-over to the APC channel. Researchers get more reliable and reproducible staining patterns, and more reliable and reproducible results.
More flexibility and greater ease of use
BD APC-H7 improves stability in ambient light, making it easier to handle and use. Experiments can be run outside darkroom conditions with more relaxed time constraints.
Conjugates of BD APC-H7 are available for many important cell surface markers such as CD4, CD8, CD14, CD20, anti-HLA-DR, CD19, CD3, and CD45, allowing BD APC-H7 to be used for a wide range of applications. This degree of choice, coupled with increased stability, enables greater flexibility in multicolor experiment design.
Addresses the spillover challenge
Emission at the donor fluorochrome wavelength, caused by dye degradation and spillover, has been a challenge in the use of tandem dyes such as APC-cyanine dyes. Degradation inherent in tandem dyes leads to emission in the APC channel, which in turn leads to false positive events and reduced sensitivity. Spillover is a common issue with multicolor applications, and can result in loss of resolution sensitivity. BD APC-H7 reduces loss of fluorescence resonance energy transfer (FRET) between the donor and acceptor fluorophores to maintain integrity of signals from both channels.
Visit bdbiosciences.com/apc-h7 for more information and a complete product listing.
Figure 2 A. APC-H7 vs. APC-Cy7: Comparison of staining stability (in BDSF, under light at RT).
Whole blood stained with CD4 APC-H7, CD8 APC-H7, CD4 APC-Cy7, or CD8 APC-Cy7. Stained samples were stored in BD Stabilizing Fixative at room temperature under ambient light for 48 hours.
Figure 2 B. APC-H7 vs. APC-Cy7: Comparison of staining stability (in 1% PFA, under light at RT).
Whole blood stained with CD4 APC-H7, CD8 APC-H7, CD4 APC-Cy7, or CD8 APC-Cy7. Stained samples were stored in 1% PFA at room temperature under ambient light for 48 hours.
