Cytotoxic Cell Assays Taken One Step Farther
Degranulating cells are identified by their surface expression of CD107a, which is a lysosomal-associated membrane protein (LAMP-1) residing in cytolytic granule membranes located within the cytoplasm. CD107a is mobilized to the cell surface following activation-induced granule exocytosis. Because of their parallel kinetics, CD107a and intracellular cytokines can be assessed at the same time in short-term-activated blood samples.
CD107a flow cytometric degranulation assay
- Designed for detection of degranulating T lymphocytes in activated whole blood
- Can be used as an alternative to 51Cr release assays
- Detects cytolytic activity of CD8+ T cells by measuring degranulation, a prerequisite for cytolysis
| Description | React | Clone | Apps | Reg | Format | Size | Cat. No. |
|---|---|---|---|---|---|---|---|
| CD107a | Hu | 1D4B | FCM | RUO(GMP) | APC | 100 tests | 641581 |
References
Betts, M, Brenchley J, Price D et al. Sensitive and viable identification of antigen-specific CD8+ T cells by flow cytometric assay for degranulation. J Immunol Met. 2003;281:65-67.