Protocols

Flow Cytometry

• General
  1. BD FACSArray™ Bioanalyzer Instrument Quality Control Instruction Manual
  2. Fluorochrome Absorption and Emission Spectra
  3. Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis
  4. Isotype Control Antibody Selection Guide
• Cytokine Analysis
  1. Cell Fixation/Permeabilization Kits for Intracellular Cytokine Analysis
  2. Activation of Immune Cells for Cytokine Production
  3. Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis
• Cell Biology
  1. Annexin V Staining Protocol
  2. APO-BRDU™ Procedure
  3. APO-DIRECT™ Procedure
  4. Detection of BrdU Incorporation in DNA Synthesizing Cells
  5. Staining Procedure for Flow Cytometric Detection of Human Cyclins
• BD™ Phosflow
  1. BD™ Phosflow Protocol for Adherent Cells
  2. BD™ Phosflow Protocol for Human PBMCs
  3. BD™ Phosflow Protocol for Human Whole Blood Samples
  4. BD™ Phosflow Protocol for Mouse Splenocytes or Thymocytes
  5. 96 Deep Well Phosflow Staining with Cytokine Stimulation
  6. 96 deep Well Phosflow Staining with TCR Stimulation
  7. Phosflow FAQ
• Human
  1. Indirect Immunofluorescence Staining of Human Platelets
  2. Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
  3. Surface Staining of Human Red Blood Cells
  4. Intracellular Staining of Human Red Blood Cells
  5. Staining Protocol for the Detection of Ki-67
  6. Cell Surface Staining of Human PBMCs and Suspension Cell Lines
• Mouse
  1. Immunofluorescent Staining of Mouse and Rat Leukocytes
  2. The Uses of Fc Block™ in Immunophenotyping of Mouse or Rat Leukocytes

Cytometric Bead Array (CBA)

• CBA instrument setup protocols and CBA kit manuals
• BD CBA Software user manual
• CBA Flex Set instrument setup manuals and Master Buffer Kit manuals
• FCAP Array Software for the analysis of CBA Flex Set assays

BioImaging

• Immunofluorescence Protocol for BD BioImaging Certified Reagents

ELISA

• Cytokine ELISA
• Mouse IgE ELISA Protocol

ELISPOT

• Cytokine ELISPOT Reagent Set manual

Immunohistochemistry

• Pharmingen Reagents
  1. Preparation and Staining of Frozen Tissue Sections
  2. Preparation and Staining of Paraffin Sections
  3. Immunohistochemical Staining of Phospho-proteins in Paraffin Sections
• Transduction Laboratories Reagents
  1. Immunohistochemistry / Tissue Section Staining
  2. Cell Staining for Immunofluorescence Microscopy

Western Blotting

• Western Blotting with Biotinylated Antibodies
• Western Blotting with Horseradish Peroxidase Conjugates and Alkaline Phosphatase Conjugates
• Western Blotting with Monoclonal Antibodies
• Western Blotting with Rabbit Polyclonal Antibodies
• Western Blotting Technical Tips

Immunoprecipitation

• Immunopurification of Tyrosine Phosphorylated Proteins
• Immunoprecipitation with Antibody: Agarose Conjugates
• Immunoprecipitation with anti-Phosphotyrosine: Biotin Conjugates
• Immunoprecipitation With Soluble Antibodies

Baculovirus Protein Expression

• Introduction to Producing and Maintaining AcNPV-derived Baculoviruses
• Generating Recombinant Viruses by Co-transfection
• End-point Dilution Assay
• Plaque Assay
• Baculovirus Amplification

RNAse Protection Assay (RPA)

• RNase Protection Assay
• Total RNA Isolation

Techniques for Induction of Apoptosis

• Cell Death Induced by Camptothecin
• Cell Death Induced by Staurosporine
• Induction of Apoptosis Using anti-human Fas (CD95), Clone DX2
• Induction of Apoptosis Using anti-mouse Fas (CD95), Clone Jo2