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The BD™ Human Pluripotent Stem Cell Sorting and Analysis
Kit provides a comprehensive system for the sorting and
characterization of pluripotent stem cells using flow cytometry.
The ready-to-use kit integrates antibodies, compensation
beads, protocols, and analysis tools to maximize reproducibility
and improve productivity. An open design allows for the easy
addition of supplementary antibodies to adapt the kit to meet
specific research objectives.
Analysis of H9 hESCs for expression of SSEA-3, TRA-1-81, and SSEA-1. The plot on the upper left shows the scatter plot of the cells established using BD CompBead Plus microparticles. Populations are shown as clusters defined by two markers.
Flow Cytometry for Multi-parameter Analysis
The Pluripotent Stem Cell Kit lets researchers quickly and reliably
sort and analyze cells by leveraging the full capabilities of flow
cytometry. A number of different parameters can be analyzed
simultaneously, enabling the identification of cell populations
based on scatter properties and the surface expression of
multiple markers for pluripotency and differentiation.
High Quality, Ready-to-Use Antibodies
Pre-titered, pre-conjugated monoclonal antibodies provided
in the kit accurately pinpoint pluripotent cells and streamline
procedures. The monoclonal antibodies are specific for two
pluripotency markers (TRA-1-81 Alexa Fluor® 647 and SSEA-3
PE) and one human differentiation marker (SSEA-1 FITC).
Corresponding isotype controls are also included.
BD CompBead Plus Enables Consistent Setup
BD™ CompBead Plus microparticles simplify experimental setup
by facilitating compensation for multicolor analysis. The use of
BD CompBead Plus significantly reduces the number of cells
required for experiments and controls while ensuring consistent
experimental setup.
Protocols and Analysis Guidelines
Protocols for processing, sorting, and recovering cells for
multiple human embryonic stem cell (hESC) lines streamline
the process and eliminate guesswork. Software template
setup guidelines simplify analysis and gating for cell sorting.
Protocols and guidelines for analysis are both designed to ensure
consistent experimental reporting.
Modular and Open to Accommodate Specific Needs
For simple customization and more advanced analysis, the open,
modular architecture of the kit allows for the easy addition of
supplementary monoclonal antibodies against critical cell-surface
markers. BD CompBead Plus microparticles can also be used as
compensation controls for additional antibody drop-ins to this
kit. This is particularly useful when testing for markers that may
or may not be expressed on your cells of interest.
A Resource for Stem Cell Research
BD Biosciences continues to support innovation in the area
of stem cell research with over 20 years of experience in the
field. Inspired by in-depth understanding of the complexities
of biological experiments, the BD Human Pluripotent Stem Cell
Sorting and Analysis Kit is designed to make it easier
for researchers to obtain accurate results, increase research
productivity, and accelerate discoveries. The data shown here
illustrate a typical experiment designed to sort pluripotent cells
from H9 hESCs.
Analysis of H9 hESCs for expression of SSEA-3, TRA-1-81, and SSEA-1. Populations are shown as histograms for single-color analysis.
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