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Stem Cell Research Source

Mesenchymal Stem Cells

The study of stem cells offers a new way to better understand the fundamental events and mechanisms regulating human health, development, and disease.

MSCs have been reported to differentiate into a variety of cell types such as osteoblasts, chondrocytes, myocytes, adipocytes, and, pancreatic beta-islets cells and neuronal cells. MSCs are multipotent stem cells, found in the bone marrow. They have also been isolated from adipose tissue, umbilical cord, synovium, amniotic fluid, and dental pulp.

Experiment results

Flow cytometric analysis of mesenchymal stem cells »

The ultimate goal of stem cell research is to develop cell-based therapies to treat conditions currently untreatable by conventional therapies. To achieve this, protocols are needed to expand, characterize, and isolate sufficient quantities of purified cell populations of a defined lineage.

Flow cytometry, combined with the development of new antibodies, can improve cell population purity and provide faster multiparametric analysis to help accelerate the pace of discovery.

Researchers suggest mesenchymal stem cells (MSCs) are thought to have promising potential in therapeutics.

Experiment Results

 

Flow cytometric analysis of mesenchymal stem cells.

Mesenchymal stem cells (MSCs) from bone marrow (Lonza) were analyzed for expression of surface markers. MSCs expressed the known positive CD29 (Cat. No. 559883), CD44 (Cat. No. 555478), and CD90 (Cat. No. 555595) markers. Markers known to be negative on MSCs—CD34 (Cat. No. 555821) and CD45 (Cat. No. 555482)—were not detected on these cells.

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